Tissue Culture


 
 
Concept Explanation
 

Tissue Culture

Tissue culture involves maintaining of tissues or organs (in case of plant tissue culture isolated cells or small pieces of plant tissues are used) under sterile or  in vitro conditions in nutrient cultures to grow into new disease-free plants. This technique is also known as in vitro micropropagation because it takes place in a laboratory and the plants are transferred from the labs to the soil.

Plant tissue culture is widely used for the rapid multiplication of plant species with superior qualities. This method involves following steps:

  • In this method, small pieces are cut from the plant like flower buds, stem tissue, growing tips, leaves, etc., (known as ex-plants).
  • These a then transferred to an artificial medium where they divide rapidly to form a callus (undifferentiated mass of cells).
  • This callus is then transferred to fresh medium containing hormones for growth and differentiation, where it subsequently develops into platelets.After this, the developed platelets are placed in the soil to grow into a mature plant.
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    Sample Questions
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    Question : 1

    Undifferentiated mass of cells is known as ___________________

    Right Option : B
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    Explanation
    Question : 2

    Tissue culture is used for rapid vegetative propagation as well as for the production of disease-free and pest-resistant plants. It is also known as ___________________.

    Right Option : B
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    Explanation
    Question : 3

    Production of an exact copy of organism is called __________________

    Right Option : B
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    Explanation
     
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